Automated Freezing Detector
46000 - NEW Fear Conditioning System
The Ugo Basile Fear Conditioning System includes all the components to run experiments on mice or rats, according to the paradigms:
- Contextual Fear Conditioning
- Cued Fear Conditioning
The detection of Freezing is automated and based on video analysis, by using the Any-Maze software (specific version for Fear Conditioning), which also controls the shocker and the sound generator.
Our Fear Conditioning system works also in total darkness. In the new model, all controls are managed by a single Electronic Unit with touch-screen.
- Features and Benefits
- AUTOMATIC detection of FREEZING also in Total Darkness
- Specific versions for rats or mice
- Multiple Cage Set-up (up to 16 cages, in groups of 4)
NEW on the 2014 version!
- The new “launcher” application, makes it possible to manage other UB behavioral cages with the same Touch-Screen Controller 40500-001; just purchase the hardware and the application software for the additional test!
- Remote Control feature will make remote service and software upgrades extremely simple!
The new FC Controller 46000-100 consolidates all controls in a single compact electronic unit.
- Sound, in the range 100 Hz-18KHz; 55-100dB or white noise. The speaker is included in the Cubicle.
- Shock: constant current (from 0.1 to 2.9 mA in 0.1mA steps). The shock can be controlled via external operation (via 5V TTL signals)
Animal Box with Electrified Grid Floor
- 46003 Mouse Box: inside dimensions: 17x17x25(h) cm
- 46002 Rat Box: inside dimensions : 26x26x30(h) cm
- Cubicle 55(d) x 60(w) x 57(h) cm
- Controller 25(d) x 33(w) x 5.5(h) cm
- Mouse Cage 21(d) x 24(w) x 30(h) cm
- Rat Cage 30(d) x 34(w) x 41.5(h) cm
- Shipping Dimensions 79x61x75 cm for each cage
- Weight 35Kg, for each cage set
- Shipping Weight 42Kg, approx. for each cage set
Fear Conditioning is a quick and reliable method to assess memory in rodents.
When rodents are exposed to fearful stimuli, they respond, among the others, with immobility behavior, also called “freezing”. All different Fear Conditioning procedures imply the measurement of the freezing response (i.e. the fraction of time spent immobile).
A rodent can learn to fear a previous neutral stimulus if that has been associated with an instinctively aversive one (such as an electric shock), or, in other terms, after having been conditioned.
After the conditioning has occurred, and the two stimuli have become associated, the animal is presented the neutral stimulus and, as a consequence, it will freeze. Freezing is commonly defined as complete immobility, apart from respiratory movements.
The time spent freezing is indicative of undergone memory and learning processes, which are at the basis of the association between neutral and aversive stimuli.
Fear Conditioning is a sensitive and quick test, which requires very little training (usually only one trial) to the animals. It is therefore a valuable tool for basic behavior research, as well as for high throughput and drug discovery studies. Moreover, the conditioning established during the Fear Conditioning procedure can be very strong (i.e. long-lasting), allowing for long term experiments.
The Ugo Basile Fear Conditioning systems are designed to run experiments of Pavlovian conditioning on mice or rats.
Single-frequency sounds are delivered as neutral or conditioned, stimulus (CS). Experimental procedures which contemplate the sound as CS are usually referred to as Cued Fear Conditioning.
Electric current from the grid floor is delivered as the naturally aversive, or unconditioned stimulus (US).
Tests are run in an animal cage with a grid floor, whose appearance can be easily altered by attaching patterned contexts on the walls and floor. This allows for the investigation of Contextual Fear Conditioning, in which the CS is not the sound but the visual appearance of the cage (i.e. the context) itself. In this case the animal associates the electric shock to the environment where it was received.
The detection of Freezing is automated and based on video analysis. The shock, light and sound parameters are controlled by software (USB) or manually, via the new Electronic Unit, based on touchscreen technology.
The context kit provided with each cage alter the animal cage appearance, allowing for both Contextual and Cued procedures to be run with the same system.
COMPLETE SYSTEMS (with software and USB camera)
- 46153 Complete Single-Cage FC System
- 46253 Complete Two-Cage FC System
- 46453 Complete Four-Cage FC System
- 46152 Complete Single-Cage FC System
- 46252 Complete Two-Cage FC System
- 46452 Complete Four-Cage FC System
- 46103 Basic Single-Cage FC System
- 46203 Basic Two-Cage FC System
- 46403 Basic Four-Cage FC System
- 46102 Basic Single-Cage FC System
- 46202 Basic Two-Cage FC System
- 46402 Basic Four-Cage FC System
- 46102-002 Rat
- 46103-003 Mouse
- S. Yusufisaq et alia: "Post-Weaning Social Isolation impairs observational fear conditioning" Behav. Brain Res. 242 (1): 142-149, 2013
- A. Sirri et al.: “Temporal gene expression profile of the hippocampus following trace fear conditioning”. Brain Research 1308 , 14-23, 2010